Journal: Tissue Engineering and Regenerative Medicine

Article Title: Perfusion Bioreactor Conditioning of Small-diameter Plant-based Vascular Grafts

doi: 10.1007/s13770-024-00670-0

Figure Lengend Snippet: Representative H&E images of grafts at 40 × magnification with A no endothelial cells (ECs) and vascular smooth muscle cells, B endothelial cells and vascular smooth muscle cells, C endothelial cells and vascular smooth muscle cells with flow, D endothelial cells and vascular smooth muscle cells with flow and pressure. E Distribution of ECM orientation. F Wall thickness, G fiber thickness and H cell wall fiber volume-fraction were measured for 3 images per sample ( N = 9 ). Error bars represent standard deviation

Article Snippet: Vascular smooth muscle cells were cultured in Smooth Muscle Cell Growth Medium (Cell Applications) and differentiated in Smooth Muscle Cell Induction Medium (Cell Applications).

Techniques: Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Biodegradable Polymers Influence the Effect of Atorvastatin on Human Coronary Artery Cells

doi: 10.3390/ijms17020148

Figure Lengend Snippet: The viability of vascular cells is influenced by polymers. Human coronary artery endothelial cells (HCAEC) and human coronary artery smooth muscle cells (HCASMC) were cultured on control and polymer surfaces for six days. Cell viability was determined after incubation with AlamarBlue ® (Biosource, Camarillo, CA, USA). Bars show mean ± SD of six separate experiments normalized to the control with * p < 0.05 compared to the HCAEC control and + p < 0.05 compared to the HCASMC control.

Article Snippet: Cells were obtained at passage 2 and cultured in endothelial cell growth medium (ECGM, Provitro GmbH, Berlin, Germany) or smooth muscle cell growth medium (SMCGM, Provitro GmbH) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA).

Techniques: Cell Culture, Control, Polymer, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Biodegradable Polymers Influence the Effect of Atorvastatin on Human Coronary Artery Cells

doi: 10.3390/ijms17020148

Figure Lengend Snippet: Ki67 expression in human coronary artery smooth muscle cells (HCASMC) after local and systemic atorvastatin (ATOR) administration. HCASMC were cultured on polycarbonate membranes and placed on ATOR-releasing PLLA/P(4HB) surfaces or PLLA/P(4HB) films. Cells were perfused at 20 dyne/cm 2 for 3 h using a parallel plate flow chamber. Perfusion was performed in basal medium supplemented with 2% FBS. Plus (+) and minus (−) indicate the presence or absence of a component indicated below the bar chart. After flow exposure, cells were detached and prepared for quantitative RT-PCR. Bars show mean ± SD of at least three separate experiments normalized to HCASMC kept under static conditions in basal medium supplemented with 2% FBS ( + p < 0.05).

Article Snippet: Cells were obtained at passage 2 and cultured in endothelial cell growth medium (ECGM, Provitro GmbH, Berlin, Germany) or smooth muscle cell growth medium (SMCGM, Provitro GmbH) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Cell Culture, Quantitative RT-PCR